RBS
Part:BBa_M50008:Design
Designed by: Cale Lester, Raquel Freeman, Tristan Yeung Group: Stanford BIOE44 - S11 (2016-10-31)
U10 Thermosensitive 5'-UTR (includes RBS)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 14
Illegal PstI site found at 20 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 14
Illegal PstI site found at 20 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 14
Illegal PstI site found at 20 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 14
Illegal PstI site found at 20 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
No BsaI sites, no palindromic repeats greater than 10bp, not over 2kbp.
Source
Our unique parts are based off of E. coli thermosensors designed by:
Neupert, J., et al. 2008. Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli. Nucleic Acids Research. 36(19): e124.